what is PCR for HLA B27 (EDTA)?

The PCR for HLA B27 (EDTA) test is a molecular diagnostic test used to detect the presence of the HLA-B27 gene, a genetic marker strongly associated with autoimmune disorders like ankylosing spondylitis, reactive arthritis, psoriatic arthritis, and uveitis.

This test is performed using Polymerase Chain Reaction (PCR) technology on a blood sample collected in an EDTA tube. Early detection can guide treatment decisions and help manage symptoms more effectively.

✨ why take the HLA B27 PCR (EDTA) test in dubai?

? Identify Genetic Predisposition: Especially for ankylosing spondylitis (AS) & related autoimmune diseases.
? Highly Accurate PCR Technique: Detects gene sequences with precision.
? Simple EDTA Blood Sample: No fasting required.
? Widely Available in Dubai: Performed by top certified diagnostic labs.
? Fast Turnaround: Results in 2–5 working days.
?‍⚕️ Supports Clinical Diagnosis: Alongside symptoms and imaging (MRI/X-ray).

common symptoms prompting this test

✔️ Chronic lower back pain
✔️ Stiffness in the spine or hips
✔️ Pain in the heels or chest
✔️ Inflammation of eyes (uveitis)
✔️ Family history of autoimmune disorders

procedure of the test

1. Sample Collection

   • A blood sample is drawn from a vein using a sterile syringe and collected in a purple-top EDTA tube.

2. PCR Analysis

   • The sample is sent to the laboratory where PCR technology amplifies the DNA and detects the HLA-B27 gene if present.

3. Results

   • The lab issues a positive or negative result indicating the presence or absence of the HLA-B27 gene.

methodology of the test

? Polymerase Chain Reaction (PCR) is a molecular biology technique that amplifies small segments of DNA to detect specific gene markers. In this test:

• DNA is extracted from white blood cells in the EDTA sample.

• The PCR machine targets the HLA-B27 gene sequence.

• If HLA-B27 is present, it gets amplified and identified.

This method offers high sensitivity, accuracy, and reliability in genetic testing.

conclusion

The PCR for HLA B27 (EDTA) test is an essential diagnostic tool for identifying a genetic predisposition to autoimmune conditions, particularly ankylosing spondylitis. If you're experiencing symptoms like chronic back pain, joint stiffness, or eye inflammation, or have a family history of autoimmune disorders, this test can help confirm your diagnosis.

? Early detection = better management & treatment planning!
? Book your HLA-B27 PCR Test in Dubai today and take charge of your health. ?