What Is HER-2/NEU – FISH Test?

The HER-2/NEU – FISH Test is a specialized genetic test used to detect HER2 gene amplification in breast cancer cells. HER2 (human epidermal growth factor receptor 2) is a protein that promotes cell growth. Overexpression or amplification of the HER2 gene can lead to aggressive breast cancer.

This test uses Fluorescence In Situ Hybridization (FISH) to visualize HER2 gene copies directly in the cancer tissue. It is crucial in determining whether a patient is eligible for targeted therapies like trastuzumab (Herceptin).

Why Choose HER2 FISH Test In Dubai?

? High Precision Testing: Accurate detection of HER2 gene status
? Guides Targeted Therapy: Helps oncologists prescribe HER2-targeted treatments
? Advanced FISH Technique: Uses fluorescent probes for exact gene visualization
? Available In Leading Labs Across Dubai
?‍⚕️ Backed By International Guidelines (ASCO, CAP)
? Results Within 7–10 Days

Who Should Get The HER-2/NEU – FISH Test?

✔️ Patients diagnosed with breast cancer (especially invasive)
✔️ Tumors with equivocal HER2 IHC (2+) results
✔️ Cases being considered for HER2-targeted therapy
✔️ Recurrent or metastatic breast cancer patients
✔️ Individuals planning for a personalized treatment plan

Benefits Of The HER2 FISH Test

✨ Confirms HER2 Status When IHC Results Are Inconclusive
✨ Identifies Eligibility For Drugs Like Trastuzumab, Pertuzumab, etc.
✨ Improves Treatment Accuracy With Personalized Oncology
✨ Minimally Invasive – Uses FFPE Tumor Tissue
✨ Globally Recognized Method For Breast Cancer Management

Procedure Of The Test

1. Tissue Sample Collection

   • The test is done on a formalin-fixed paraffin-embedded (FFPE) tissue sample, usually collected during a biopsy or surgery.

2. Lab Processing

   • A thin section of the tissue is prepared on a glass slide for FISH analysis.

3. Fluorescent Probe Hybridization

   • Special fluorescent DNA probes are applied to the sample to bind with the HER2 gene region.

4. Microscopic Examination

   • A pathologist uses a fluorescence microscope to count the number of HER2 gene copies and chromosome 17 (reference probe) signals.

5. Result Interpretation

   • HER2 amplification is confirmed if the HER2/CEP17 ratio ≥ 2.0 or average HER2 signals ≥ 6 per cell.

Methodology Of The Test

The HER-2/NEU – FISH Test utilizes the Fluorescence In Situ Hybridization technique:

? DNA Probes labeled with fluorescent dyes are designed to bind specifically to the HER2 gene locus and a reference point (usually chromosome 17 centromere).

? Under a fluorescence microscope:

• HER2 gene copies appear as bright red signals

• Chromosome 17 appears as green signals

? The number of signals is counted in at least 20 cells to determine gene amplification status.

This method is highly accurate, even when Immunohistochemistry (IHC) is inconclusive.

Conclusion

The HER-2/NEU – FISH Test is a gold standard for confirming HER2 gene amplification in breast cancer. It provides essential information for tailoring personalized treatment, especially in identifying whether HER2-targeted therapy is beneficial.

? In Dubai, access to world-class laboratories and experienced oncologists ensures accurate testing and result interpretation.
? If you or your loved one is navigating breast cancer treatment, HER2 FISH testing offers clarity and confidence in your treatment journey. ?